doshisha_commonheader
  1. Home
  2.  > Faculty
  3.  > Systems Neuroscience (Laboratory of Neural Circuitry)

Systems Neuroscience

Laboratory of Neural Circuitry

Fumino Fujiyama, M.D., Ph.D.
The basal ganglia, a group of interconnected brain areas located deep in the cerebral cortex, have proved to be at work in motor execution, learning, the formation of good and bad habits, and some psychiatric and addictive disorders.

Our laboratory aims to provide new insights into the principles of operation of the neuronal networks that constitute the basal ganglia by combined morphological, molecular biological or physiological approaches. For example, we employ the viral vectors expressing membrane-targeted green fluorescent protein (GFP) for single neuron tracing, the transgenic animals with the dendritic membrane-targeted GFP or immunohistochemistry of vesicular glutamate transporters to visualize the specific neuronal groups and post-embedding method to discover the receptors at synaptic structures. These techniques help to develop the new approaches to the functional design of the neural circuits.

The new knowledge will provide a basis for understanding how the basal ganglia influence cognitive as well as motor functions and how the network or synaptic organization are disrupted in disorders affecting the basal ganglia.
Fumino Fujiyama, M.D., Ph.D.
Fig.1. GABAergic Receptor identified by Post-embedding Method
Fig.2. Single Neuron Tracing of Striatal Neurons

Research topics

  1. draw the neural circuitry of the cortico-basal ganglia loops
  2. elucidate the local circuits of GABAergic or cholinergic interneurones in the neostriatum
  3. characterize the pathways of information flow using experimental models of basal ganglia disorders

Selected publications

  1. Mizutani K, Takahashi S, Okamoto S, Karube F, Fujiyama F. (2017) Substance P effects exclusively on prototypic neurons in mouse globus pallidus. Brain Structure and Function. in press.
  2. Oh Y-M, Karube F, Takahashi S, Kobayashi K, Takada M, Uchigashima M, Watanabe M, Nishizawa K, Kobayashi K, Fujiyama F. (2017) Using a novel PV-Cre rat model to characterize pallidonigral cells and their terminations. Brain Structure and Function. in press.
  3. Unzai T, Kuramoto E, Kaneko T, Fujiyama F. (2017) Quantitative Analyses of the Projection of individual Neurons from the Midline Thalamic Nuclei to the Striosome and Matrix Compartments of the Rat Striatum. Cerebral Cortex. 27:1164-118
  4. Fujiyama F, Nakano T, Matsuda W, Furuta T, Udagawa J, Kaneko T. (2016) A Single-Neuron Tracing Study of Arkypallidal and Prototypic Neurons in Healthy Rats. Brain Structure and Function. 9: 4733-4740
  5. Fujiyama F, Karube F, Takahashi S. (2015) Morphological elucidation of basal ganglia circuits contributing reward prediction. Frontiers in Neuroscience. 9:1-8
  6. Fujiyama F, Sohn J, Nakano T, Furuta T, Nakamura KC, Matsuda W, Kaneko T. (2011) Exclusive and common targets of neostriatofugal projections of rat striosome neurons: A single neuron-tracing study using a viral vector. Eur. J. Neurosci. 33, 668-677.
  7. Fujiyama F, Unzai T, Nakamura K, Nomura S, Kaneko T. (2006) Difference in organization of corticostriatal and thalamostriatal synapses between patch and matrix compartments of rat striatum. Eur. J. Neurosci. 24, 2813-2824.
  8. Fujiyama F., Kuramoto E., Okamoto K., Hioki H., Furuta T., Zhou L., Nomura S. & Kaneko T. (2004) Presynaptic Localization of AMPA-type Glutamate Receptor in Corticostriatal and Thalamostriatal Axon Terminals. Eur. J. Neurosci. 20, 3322-3330.
  9. Fujiyama F., Furuta T. & Kaneko T. (2001) Immunocytochemical localization of candidates for vesicular glutamate transporters in the rat cerebral cortex. J. Comp. Neurol. 435, 379-387.
  10. Fujiyama F., Fritschy J.M., Stephenson F.A. & Bolam J.P. (2000) Synaptic localization of GABA(A) receptor subunits in the striatum of the rat. J. Comp. Neurol. 416, 158-172.

Members

Fumino Fujiyama, M.D., Ph.D. (Principal investigator, Professor)
Fuyuki Karube, Ph.D. (Research associate professor)
Yasuharu Hirai, Ph.D. (Research assistant professor)

Contact

1-3 Tatara Miyakodani, Kyotanabe-shi, Kyoto 610-0394 Japan
E-mail : E-mail_fujiyama
.